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Fine-tuning formulation and biological interaction of doxorubicin-loaded polymeric nanoparticles via electrolyte concentration modulation

dc.contributorUniversitat Ramon Llull. Facultat de Ciències de la Salut Blanquerna
dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorBofill Bonet, Carles
dc.contributor.authorGil Vives, Maria
dc.contributor.authorArtigues Cladera, Margalida
dc.contributor.authorHernández Hernández, Marta
dc.contributor.authorBorrós i Gómez, Salvador
dc.contributor.authorFornaguera Puigvert, Cristina
dc.date.accessioned2024-02-04T19:59:32Z
dc.date.created2023-06
dc.date.issued2023-09
dc.identifier.urihttp://hdl.handle.net/20.500.14342/3856
dc.description.abstractDoxorubicin (DOX) is amongst the most widely used chemotherapeutic drugs against various cancers. However, its controlled biodistribution to the tumor site at the required pharmacokinetics profile remains challenging. In this work, a vast study of formulation parameters has been performed to control doxorubicin loading into polymeric nanoparticles in physiological conditions. Water-in-oil-in-water (W1/O/W2) template emulsions have been formulated, including doxorubicin in the internal water phase. Specifically, the system [Doxorubicin in aqueous solution (W1) / Pluronic F-127 (S) and PLGA in ethyl acetate (O)], [Polysorbate 80 (S) in water solution], using phosphate buffer on both aqueous phases at different electrolyte concentrations has been established. The first W1/O nano-emulsion was formed by high-speed homogenization, and the second O/W2 nano-emulsion was formed by the phase inversion composition method, a low-energy emulsification appropriated for pharmaceutical compounds. Although many previous studies had already used double emulsions for DOX encapsulation in nanofluids, here, for the first time, the salt concentration of the water phases has been varied to control the resulting doxorubicin and nanoparticle properties. The addition of high concentrations of electrolytes in both aqueous phases decreases the solubility and dissolution rates of the drug, leading to a high degree of DOX dimerization. This phenomenon significantly impacts the internalization and subcellular localization. Facilitating drug nuclear accumulation is of vital interest, and this is primarily achieved when doxorubicin is incorporated into nanoparticles formulated with PBS at low electrolyte concentrations (2.5 mM), which exhibit exceptional internalization properties. Thus, it has been demonstrated that doxorubicin loading in polymeric nanoparticles can be tuned by only varying salt concentrations of template W1/O/W2 emulsions. Consequently, our results show the adequacy of the double nano-emulsion templating for the generation of non-toxic DOX-loaded polymeric nanoparticles with different physicochemical properties and interaction with the cell surface that would allow their use for various anticancer therapies.ca
dc.format.extent12 p.ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofJournal of Molecular Liquids, 2023, vol. 390, part A, 122986ca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights© Elsevier
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.otherEmulsionsca
dc.subject.otherFarmàciaca
dc.subject.otherNanopartículesca
dc.subject.otherNanomedicinaca
dc.subject.otherDuxorubicinaca
dc.subject.otherCàncer -- Tractamentca
dc.subject.otherWater-in-oil-in-water emulsionsca
dc.subject.otherDouble emulsionsca
dc.subject.otherNano-emulsionsca
dc.subject.otherPolymeric nanoparticlesca
dc.subject.otherDoxorubicin encapsulationca
dc.subject.otherAntitumor therapiesca
dc.titleFine-tuning formulation and biological interaction of doxorubicin-loaded polymeric nanoparticles via electrolyte concentration modulationca
dc.titleFine-tuning formulation and biological interaction of doxorubicin-loaded polymeric nanoparticles via electrolyte concentration modulation
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/embargoedAccess
dc.date.embargoEnd2025-08-31T02:00:00Z
dc.embargo.terms24 mesosca
dc.subject.udc615ca
dc.subject.udc616ca
dc.identifier.doihttps://doi.org/10.1016/j.molliq.2023.122986ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICIN-AEI/PID2021-125910OB-I00ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICIN-AEI/10.13039/501100011033ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/SUR del DEC/SGR/2021 SGR 00537ca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/
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