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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorPlanas, Antoni (Planas Sauter)
dc.contributor.authorBiarnés Fontal, Xevi
dc.contributor.authorHamer, Stefanie Nicole
dc.contributor.authorCord-Landwehr, Stefan
dc.contributor.authorWaegeman, Hendrik
dc.contributor.authorMoerschbacher, Bruno Maria
dc.contributor.authorKolkenbrock, Stephan
dc.date.accessioned2020-07-03T11:19:32Z
dc.date.accessioned2023-07-13T05:44:05Z
dc.date.available2020-07-03T11:19:32Z
dc.date.available2023-07-13T05:44:05Z
dc.date.issued2015-03
dc.identifier.urihttp://hdl.handle.net/20.500.14342/1017
dc.description.abstractChitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. These properties may depend not only on the degrees of polymerization and acetylation, but also on a specific pattern of acetylation (PA) that cannot be controlled when the oligomers are produced by chemical hydrolysis. To determine the influence of the PA on the biological activities, defined chitosan oligomers in sufficient amounts are needed. Chitosan oligomers with specific PA can be produced by enzymatic deacetylation of chitin oligomers, but the diversity is limited by the low number of chitin deacetylases available. We have produced specific chitosan oligomers which are deacetylated at the first two units starting from the non-reducing end by the combined use of two different chitin deacetylases, namely NodB from Rhizobium sp. GRH2 that deacetylates the first unit and COD from Vibrio cholerae that deacetylates the second unit starting from the non-reducing end. Both chitin deacetylases accept the product of each other resulting in production of chitosan oligomers with a novel and defined PA. When extended to further chitin deacetylases, this approach has the potential to yield a large range of novel chitosan oligomers with a fully defined architecture.eng
dc.format.extent9 p.cat
dc.language.isoengcat
dc.publisherNature Researchcat
dc.relation.ispartofScientific Reports. Vol.5, 8716 (2015)cat
dc.rightsAttribution 4.0 International
dc.rights© L'autor/a
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherBiopolímerscat
dc.subject.otherOligòmerscat
dc.subject.otherMicrobiologia industrialcat
dc.subject.otherIndustrial microbiologycat
dc.subject.otherBiopolymerscat
dc.subject.otherChitosan oligomerscat
dc.titleEnzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylasescat
dc.typeinfo:eu-repo/semantics/articlecat
dc.typeinfo:eu-repo/semantics/publishedVersioncat
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapcat
dc.subject.udc579
dc.identifier.doihttps://doi.org/10.1038/srep08716cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP6/EIB.10.042cat


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/
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