Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases
Author
Planas, Antoni (Planas Sauter)
Biarnés Fontal, Xevi
Hamer, Stefanie Nicole
Cord-Landwehr, Stefan
Waegeman, Hendrik
Moerschbacher, Bruno Maria
Kolkenbrock, Stephan
Other authors
Universitat Ramon Llull. IQS
Publication date
2015-03Abstract
Chitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. These properties may depend not only on the degrees of polymerization and acetylation, but also on a specific pattern of acetylation (PA) that cannot be controlled when the oligomers are produced by chemical hydrolysis. To determine the influence of the PA on the biological activities, defined chitosan oligomers in sufficient amounts are needed. Chitosan oligomers with specific PA can be produced by enzymatic deacetylation of chitin oligomers, but the diversity is limited by the low number of chitin deacetylases available. We have produced specific chitosan oligomers which are deacetylated at the first two units starting from the non-reducing end by the combined use of two different chitin deacetylases, namely NodB from Rhizobium sp. GRH2 that deacetylates the first unit and COD from Vibrio cholerae that deacetylates the second unit starting from the non-reducing end. Both chitin deacetylases accept the product of each other resulting in production of chitosan oligomers with a novel and defined PA. When extended to further chitin deacetylases, this approach has the potential to yield a large range of novel chitosan oligomers with a fully defined architecture.
Document Type
Article
Published version
Language
English
Subject (CDU)
579 - Microbiology
Keywords
Biopolímers
Oligòmers
Microbiologia industrial
Industrial microbiology
Biopolymers
Chitosan oligomers
Pages
9 p.
Publisher
Nature Research
Is part of
Scientific Reports. Vol.5, 8716 (2015)
Grant agreement number
info:eu-repo/grantAgreement/EC/FP6/EIB.10.042
This item appears in the following Collection(s)
Rights
© L'autor/a
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/