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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorBorrós i Gómez, Salvador
dc.contributor.authorRamos Pérez, Víctor
dc.contributor.authorGutiérrez, Jordán
dc.contributor.authorIzpisúa Belmonte, J. C.
dc.contributor.authorMontserrat, Núria
dc.contributor.authorGarreta, Elena
dc.contributor.authorGonzález, Federico
dc.contributor.authorEguizábal, Cristina
dc.date.accessioned2020-12-01T16:21:24Z
dc.date.accessioned2023-07-13T05:43:43Z
dc.date.available2020-12-01T16:21:24Z
dc.date.available2023-07-13T05:43:43Z
dc.date.issued2011-04
dc.identifier.urihttp://hdl.handle.net/20.500.14342/987
dc.description.abstractReprogramming of somatic cells to induced pluripotent stem (iPS) cells can be achieved by the delivery of a combination of transcription factors, including Oct4, Sox2, Klf4, and c-Myc. Retroviral and lentiviral vectors are commonly used to express these four reprogramming factors separately and obtain reprogrammed iPS cells. Although efficient and reproducible, these approaches involve the time-consuming and labor-intensive production of retroviral or lentiviral particles together with a high risk of working with potentially harmful viruses overexpressing potent oncogenes, such as c-Myc. Here, we describe a simple method to produce bona fide iPS cells from human fibroblasts using poly-β-amino esters as the transfection reagent for the delivery of a single CAG-driven polycistronic plasmid expressing Oct4, Sox2, Klf4, c-Myc, and a GFP reporter gene (OSKMG). We demonstrate for the first time that poly-β-amino esters can be used to deliver a single polycistronic reprogramming vector into human fibroblasts, achieving significantly higher transfection efficiency than with conventional transfection reagents. After a protocol of serial transfections using poly-β-amino esters, we report a simple methodology to generate human iPS cells from human fibroblasts avoiding the use of viral vectors.eng
dc.format.extent13 p.cat
dc.language.isoengcat
dc.publisherThe American Society for Biochemistry and Molecular Biologycat
dc.relation.ispartofJournal of Biological Chemistry. Vol.286,n.14 (2011),p.12417-12428cat
dc.rights© The American Society for Biochemistry and Molecular Biology. Tots els drets reservats
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherGenètica humanacat
dc.subject.otherPoliaminescat
dc.subject.otherProteïnes--Síntesicat
dc.subject.otherCèl·lules marecat
dc.subject.otherNanopartículescat
dc.subject.otherEsterscat
dc.subject.otherTransfecciócat
dc.subject.otherHuman geneticscat
dc.subject.otherPolyaminerscat
dc.subject.otherProtein synthesiscat
dc.subject.otherStem cellscat
dc.subject.otheriPS cellscat
dc.subject.otherReprogrammingcat
dc.subject.otherTransfectioncat
dc.titleSimple generation of human induced pluripotent stem cells using poly-β-amino esters as the non-viral gene delivery systemcat
dc.typeinfo:eu-repo/semantics/articlecat
dc.typeinfo:eu-repo/semantics/publishedVersioncat
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapcat
dc.subject.udc576
dc.identifier.doihttps://doi.org/10.1074/jbc.m110.168013cat


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