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Detection of peptide-based nanoparticles in blood plasma by ELISA

dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorBorrós i Gómez, Salvador
dc.contributor.authorBode, Gerard H.
dc.contributor.authorPickl, Karin E.
dc.contributor.authorSánchez Purrà, Maria
dc.contributor.authorAlbaiges, Berta
dc.contributor.authorPötgens, Andy J. G.
dc.contributor.authorSchmitz, Christoph
dc.contributor.authorSinner, Frank M.
dc.contributor.authorLosen, Mario
dc.contributor.authorSteinbusch, Harry W. M.
dc.contributor.authorFrank, Hans-Georg
dc.contributor.authorMartinez-Martinez, Pilar
dc.date.accessioned2020-12-23T11:32:25Z
dc.date.accessioned2023-07-13T05:43:42Z
dc.date.available2020-12-23T11:32:25Z
dc.date.available2023-07-13T05:43:42Z
dc.date.issued2015-05
dc.identifier.urihttp://hdl.handle.net/20.500.14342/986
dc.description.abstractAims The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL.We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.eng
dc.format.extent12 p.cat
dc.language.isoengcat
dc.publisherPublic Library of Science (PLoS)cat
dc.relation.ispartofPLoS ONE. Vol.10, n.5 (2015), e0126136cat
dc.rightsAttribution 4.0 International
dc.rights© L'autor/a
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherNanopartículescat
dc.subject.otherPlasma sanguinicat
dc.subject.otherEnzimoimmunoassaigcat
dc.subject.otherInjeccions intravenosescat
dc.subject.otherCromatografia de líquidscat
dc.subject.otherEspectrometria de massescat
dc.subject.otherNanoparticlescat
dc.subject.otherBlood plasmacat
dc.subject.otherBiotincat
dc.subject.otherEnzyme-linked immunoassayscat
dc.subject.otherWistar ratscat
dc.subject.otherAcrylicscat
dc.subject.otherIntravenous injectionscat
dc.subject.otherLiquid chromatography-mass spectrometrycat
dc.titleDetection of peptide-based nanoparticles in blood plasma by ELISAcat
dc.typeinfo:eu-repo/semantics/articlecat
dc.typeinfo:eu-repo/semantics/publishedVersioncat
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapcat
dc.subject.udc539
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0126136cat


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Attribution 4.0 International
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