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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorNonell, Santi
dc.contributor.authorPérez-Laguna, Vanesa
dc.contributor.authorPérez-Artiaga, Luna
dc.contributor.authorLampaya-Pérez, Verónica
dc.contributor.authorGarcía-Luque, Isabel
dc.contributor.authorBallesta, Sofía
dc.contributor.authorPaz-Cristobal, Manuel P.
dc.contributor.authorGilaberte, Yolanda
dc.contributor.authorRezusta, Antonio
dc.date.accessioned2021-01-12T13:00:59Z
dc.date.accessioned2023-07-13T05:43:35Z
dc.date.available2021-01-12T13:00:59Z
dc.date.available2023-07-13T05:43:35Z
dc.date.issued2017-05
dc.identifier.urihttp://hdl.handle.net/20.500.14342/976
dc.description.abstractAntibiotic treatments frequently fail due to the development of antibiotic resistance, underscoring the need for new treatment strategies. Antimicrobial photodynamic therapy (aPDT) could constitute an alternative therapy. In bacterial suspensions of Staphylococcus aureus, which is commonly implicated in cutaneous and mucosal infections, we evaluated the in vitro efficacy of aPDT, using the photosensitizing agents rose bengal (RB) or methylene blue (MB), alone or combined with the antibiotics mupirocin (MU) or linezolid (LN). RB or MB, at concentrations ranging from 0.03 to 10 μg/ml, were added to S. aureus ATCC 29213 suspensions containing >108 cells/ml, in the absence or presence of MU or LN (1 or 10 μg/ml). Suspensions were irradiated with a white metal halide (λ 420–700 nm) or light-emitting diode lamp (λ 515 and λ 625 nm), and the number of viable bacteria quantified by counting colony-forming units (CFU) on blood agar. Addition of either antibiotic had no significant effect on the number of CFU/ml. By contrast, RB-aPDT and MB-aPDT effectively inactivated S. aureus, as evidenced by a 6 log10 reduction in bacterial growth. In the presence of MU or LN, the same 6 log10 reduction was observed in response to aPDT, but was achieved using significantly lower concentrations of the photosensitizers RB or MB. In conclusion, the combination of MU or LN and RB/MB-aPDT appears to exert a synergistic bactericidal effect against S. aureus in vitro.eng
dc.format.extent9 p.cat
dc.language.isoengcat
dc.publisherFrontiers Mediacat
dc.relation.ispartofFrontiers in Microbiology. Vol. 8 (2017), 1002cat
dc.rightsAttribution 4.0 International
dc.rights© L'autor/a
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherEstafilococs dauratscat
dc.subject.otherAntibiòticscat
dc.subject.otherFototeràpiacat
dc.subject.otherStaphylococcus aureuscat
dc.subject.otherAntibioticscat
dc.subject.otherRose Bengalcat
dc.subject.otherMethylene bluecat
dc.subject.otherPhotoinactivationcat
dc.titleBactericidal effect of photodynamic therapy, alone or in combination with mupirocin or linezolid, on Staphylococcus aureuscat
dc.typeinfo:eu-repo/semantics/articlecat
dc.typeinfo:eu-repo/semantics/publishedVersioncat
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapcat
dc.subject.udc615
dc.identifier.doihttps://doi.org/10.3389/fmicb.2017.01002cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/PN I+D/CTQ2013-48767-C3-1-Rcat
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/PN I+D/CTQ2013-48767-C3-2-Rcat


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/
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