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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorFandos, Diana
dc.contributor.authorBosch, Irene
dc.contributor.authorReddy, Ankita
dc.contributor.authorDe Puig, Helena
dc.contributor.authorLudert, Juan E.
dc.contributor.authorPerdomo-Celis, Federico
dc.contributor.authorNarváez, Calos F.
dc.contributor.authorVersiani, Alice
dc.contributor.authorNogueira, Mauricio L.
dc.contributor.authorSingla, Mohit
dc.contributor.authorLodha, Rakesh
dc.contributor.authorMedigeshi, Guruprasad R.
dc.contributor.authorLorenzana, Ivette
dc.contributor.authorRalde, Hugo Vicente
dc.contributor.authorGelvez-Ramirez, Margarita
dc.contributor.authorVillar, Luis A.
dc.contributor.authorHiley, Megan
dc.contributor.authorMendoza, Laura
dc.contributor.authorSalcedo, Nol
dc.contributor.authorHerrera, Bobby Brooke
dc.contributor.authorGehrke, Lee
dc.date.accessioned2022-03-07T16:39:59Z
dc.date.accessioned2023-07-13T05:43:30Z
dc.date.available2022-03-07T16:39:59Z
dc.date.available2023-07-13T05:43:30Z
dc.date.issued2020-06
dc.identifier.urihttp://hdl.handle.net/20.500.14342/970
dc.description.abstractBackground Dengue virus (DENV) infections pose one of the largest global barriers to human health. The four serotypes (DENV 1–4) present different symptoms and influence immune response to subsequent DENV infections, rendering surveillance, risk assessments, and disease control particularly challenging. Early diagnosis and appropriate clinical management is critical and can be achieved by detecting DENV nonstructural protein 1 (NS1) in serum during the acute phase. However, few NS1-based tests have been developed that are capable of differentiating DENV serotypes and none are currently commercially available. Methodology/Principle findings We developed an enzyme-linked immunosorbent assay (ELISA) to distinguish DENV-1-4 NS1 using serotype-specific pairs of monoclonal antibodies. A total of 1,046 antibodies were harvested from DENV-immunized mice and screened for antigen binding affinity. ELISA clinical performance was evaluated using 408 polymerase chain reaction-confirmed dengue samples obtained from patients in Brazil, Honduras, and India. The overall sensitivity of the test for pan-DENV was 79.66% (325/408), and the sensitivities for DENV-1-4 serotyping were 79.1% (38/48), 80.41% (78/97), 100% (45/45), and 79.6% (98/123), respectively. Specificity reached 94.07–100%. Significance Our study demonstrates a robust antibody screening strategy that enabled the development of a serotype NS1-based ELISA with maximized specific and sensitive antigen binding. This sensitive and specific assay also utilized the most expansive cohort to date, and of which about half are from Latin America, a geographic region severely underrepresented in previous similar studies. This ELISA test offers potential enhanced diagnostics during the acute phase of infection to help guide patient care and disease control. These results indicate that this ELISA is a promising aid in early DENV-1-4 diagnosis and surveillance in regions of endemicity in addition to offer convenient monitoring for future vaccine interventions.eng
dc.format.extent15 p.cat
dc.language.isoengcat
dc.publisherPublic Library of Science (PLoS)cat
dc.relation.ispartofPLoS Neglected Tropical Diseases. Vol. 14, n.6 (2020), e0008203cat
dc.rightsAttribution 4.0 International
dc.rights© L'autor/a
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherDenguecat
dc.subject.otherImmunoassaigcat
dc.subject.otherImmunoglobulinescat
dc.subject.otherDengue viruscat
dc.subject.otherEnzyme-linked immunoassayscat
dc.subject.otherAntibodiescat
dc.subject.otherDengue fevercat
dc.subject.otherBrazilcat
dc.subject.otherHondurascat
dc.subject.otherIndiacat
dc.subject.otherEnzyme assayscat
dc.titleSerotype-specific detection of dengue viruses in a nonstructural protein 1-based enzyme-linked immunosorbent assay validated with a multinational cohortcat
dc.typeinfo:eu-repo/semantics/articlecat
dc.typeinfo:eu-repo/semantics/publishedVersioncat
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapcat
dc.subject.udc616
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0008203cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/FAPESP/Grant 3/21719-3cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/FAPESP/Fellowship/Grant 2018/17647-0cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/Wellcome trust-DBT India Alliance/IA/S/14/1/501291cat


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