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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorCaballé, Marc
dc.contributor.authorFaijes, Magda
dc.contributor.authorPlanas, Antoni (Planas Sauter)
dc.date.accessioned2024-10-22T12:25:13Z
dc.date.available2024-10-22T12:25:13Z
dc.date.issued2022
dc.identifier.issn1422-0067ca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/4454
dc.description.abstractGlycolipids are complex molecules involved in important cellular processes. Among them, the glycosphingolipid α-galactosylceramide has proven to be of interest in biomedicine for its immunostimulatory capabilities. Given its structural requirements, the use of ceramide glycosyltransferase enzymes capable of synthesizing this molecule under in vivo or in vitro conditions is a potential production strategy. Several GT4 enzymes from Bacteroides fragilis were considered as potential candidates in addition to the known BF9343_3149, but only this one showed glycolipid synthase activity. The enzyme was expressed as a SUMO fusion protein to produce soluble protein. It is a non-processive glycosyltransferase that prefers UDP-Gal over UDP-Glc as a donor substrate, and maximum activity was found at pH 7.3 and around 30–35 °C. It does not require metal cations for activity as other GT4 enzymes, but Zn2+ inactivates the enzyme. The reaction occurs when the ceramide lipid acceptor is solubilized with BSA (100% conversion) but not when it is presented in mixed micelles, and anionic lipids do not increase activity, as in other membrane-associated glycolipid synthases. Further protein engineering to increase stability and activity can make feasible the enzymatic synthesis of α-GalCer for biomedical applications.ca
dc.format.extentp.16ca
dc.language.isoengca
dc.relation.ispartofInternational Journal of Molecular Sciences 2022, 23(22), 13975ca
dc.rights© L'autor/aca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherGlycosyltransferaseca
dc.subject.otherGlycosphingolipidsca
dc.subject.otherGalactosylceramideca
dc.subject.otherGlucosiltransferasesca
dc.subject.otherGlicosfingolípidsca
dc.titleCharacterization of a Glycolipid Synthase Producing α-Galactosylceramide in Bacteroides fragilisca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc577ca
dc.identifier.doihttps://doi.org/10.3390/ijms232213975ca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca


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