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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorFornaguera Puigvert, Cristina
dc.contributor.authorLázaro, Miguel Ángel
dc.contributor.authorBrugada Vilà, Pau
dc.contributor.authorPorcar, Irene
dc.contributor.authorMorera, Ingrid
dc.contributor.authorGuerra Rebollo, Marta
dc.contributor.authorGarrido, Cristina
dc.contributor.authorBlanco, Jeronimo
dc.contributor.authorCascante, Anna
dc.contributor.authorBorrós i Gómez, Salvador
dc.date.accessioned2024-09-06T07:09:16Z
dc.date.available2024-09-06T07:09:16Z
dc.date.issued2018-02-07
dc.identifier.issn1521-0464ca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/4393
dc.description.abstractGlioblastoma multiforme (GBM) is the most devastating primary brain tumor due to its infiltrating and diffuse growth characteristics, a situation compounded by the lack of effective treatments. Currently, many efforts are being devoted to find novel formulations to treat this disease, specifically in the nanomedicine field. However, due to the lack of comprehensive characterization that leads to insufficient data on reproducibility, only a reduced number of nanomedicines have reached clinical phases. In this context, the aim of the present study was to use a cascade of assays that evaluate from physical-chemical and structural properties to biological characteristics, both in vitro and in vivo, and also to check the performance of nanoparticles for glioma therapy. An amphiphilic block copolymer, composed of polyester and poly(ethylene glycol; PEG) blocks, has been synthesized. Using a mixture of this copolymer and a polymer containing an active targeting moiety to the Blood Brain Barrier (BBB; Seq12 peptide), biocompatible and biodegradable polymeric nanoparticles have been prepared and extensively characterized. In vitro studies demonstrated that nanoparticles are safe for normal cells but cytotoxic for cancer cells. In vivo studies in mice demonstrated the ability of the Seq12 peptide to cross the BBB. Finally, in vivo efficacy studies using a human tumor model in SCID mice resulted in a significant 50% life-span increase, as compared with non-treated animals. Altogether, this assay cascade provided extensive pre-clinical characterization of our polymeric nanoparticles, now ready for clinical evaluation.ca
dc.format.extent12 p.ca
dc.language.isoengca
dc.publisherTaylor & Francisca
dc.relation.ispartofDrug Deliveryca
dc.rights© L'autor/aca
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherGlioblastoma multiformeca
dc.subject.otherNanoparticle preclinical characterizationca
dc.subject.otherNCL assay cascade protocolca
dc.subject.otherPolymeric nanoparticlesca
dc.subject.otherPaclitaxelca
dc.subject.otherResearch lab results translationca
dc.subject.otherGlioblastoma multiformeca
dc.subject.otherNanopartículesca
dc.titleApplication of an assay Cascade methodology for a deep preclinical characterization of polymeric nanoparticles as a treatment for gliomasca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc539ca
dc.subject.udc615ca
dc.identifier.doihttps://doi.org/10.1080/10717544.2018.1436099ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO i FEDER/PN I+D/RTC-2015-3751-1ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO i FEDER/PN I+D/SAF2015-64927-C2-1-Rca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO i FEDER/PN I+D/SAF2015-64927-C2-2-Rca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca


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