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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorMiró-Vinyals, Bernat
dc.contributor.authorArtigues Cladera, Margalida
dc.contributor.authorWostrikoff, Katia
dc.contributor.authorMonte, Elena
dc.contributor.authorBroto Puig, Francesc
dc.contributor.authorLeivar Rico, Pablo
dc.contributor.authorPlanas, Antoni (Planas Sauter)
dc.date.accessioned2024-06-17T19:18:14Z
dc.date.available2024-06-17T19:18:14Z
dc.date.issued2023-04-11
dc.identifier.issn1871-6784ca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/4117
dc.description.abstractHydroxyalkanoyloxyalkanoates (HAA) are lipidic surfactants with a number of potential applications, but more remarkably, they are the biosynthetic precursors of rhamnolipids (RL), which are preferred biosurfactants thanks to their excellent physicochemical properties, biological activities, and environmental biodegradability. Because the natural highest producer of RLs is the pathogenic bacterium Pseudomonas aeruginosa, important efforts have been dedicated to transfer production to heterologous non-pathogenic microorganisms. Unicellular photosynthetic microalgae are emerging as important hosts for sustainable industrial biotechnology due to their ability to transform CO2 efficiently into biomass and bioproducts of interest. Here, we have explored the potential of the eukaryotic green microalgae Chlamydomonas reinhardtii as a chassis to produce RLs. Chloroplast genome engineering allowed the stable functional expression of the gene encoding RhlA acyltransferase from P. aeruginosa, an enzyme catalyzing the condensation of two 3-hydroxyacyl acid intermediaries in the fatty acid synthase cycle, to produce HAA. Four congeners of varying chain lengths were identified and quantified by UHPLC-QTOF mass spectrometry and gas chromatography, including C10-C10 and C10-C8, and the less abundant C10-C12 and C10-C6 congeners. HAA was present in the intracellular fraction, but also showed increased accumulation in the extracellular medium. Moreover, HAA production was also observed under photoautotrophic conditions based on atmospheric CO2. These results establish that RhlA is active in the chloroplast and is able to produce a new pool of HAA in a eukaryotic host. Subsequent engineering of microalgal strains should contribute to the development of an alternative clean, safe and cost-effective platform for the sustainable production of RLs.ca
dc.format.extent12 p.ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofNew Biotechnologyca
dc.rights© L'autor/aca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.otherRhamnolipid biosurfactantsca
dc.subject.otherHAA precursorca
dc.subject.otherRhlA acyltransferaseca
dc.subject.otherMicroalgae Chlamydomonas reinhardtiica
dc.subject.otherChloroplast transformationca
dc.subject.otherMetabolic Engineeringca
dc.subject.otherAgents tensioactiusca
dc.subject.otherQuímica orgànicaca
dc.subject.otherPseudomones aeruginosaca
dc.titleChloroplast engineering of the green microalgae Chlamydomonas reinhardtii for the production of HAA, the lipid moiety of rhamnolipid biosurfactantsca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc547ca
dc.identifier.doihttps://doi.org/10.1016/j.nbt.2023.03.005ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN/PN I+D/PID2019-104350RB-I00ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/SUR del DEC/SGR/2017SGR-727ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/URL i La Caixa/DYNAMO ANR-11-LABX-0011–01ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIU i FEDER/PN I+D/PGC2018–099987-B-I00ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIU i FEDER/PN I+D/PID2021–122288NB-I00ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/SUR del DEC/SGR/2017SGR-718ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC/Centro de Excelencia Severo Ochoa Program/CEX2019–000902-Sca
dc.relation.projectIDinfo: eu-repo/grantAgreement/SUR del DEC i FSE/FI/2018FI_B00823ca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca


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