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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorRovira, Arnau
dc.contributor.authorSentandreu, Maria
dc.contributor.authorNagatani, Akira
dc.contributor.authorLeivar Rico, Pablo
dc.contributor.authorMonte, Elena
dc.date.accessioned2024-05-23T19:55:45Z
dc.date.available2024-05-23T19:55:45Z
dc.date.issued2021-03-09
dc.identifier.issn1664-462Xca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/4077
dc.description.abstractDuring seedling etiolation after germination in the dark, seedlings have closed cotyledons and form an apical hook to protect the meristem as they break through the soil to reach the surface. Once in contact with light, the hook opens and cotyledons are oriented upward and separate. Hook development in the dark after seedling emergence from the seed follows three distinctly timed and sequential phases: formation, maintenance, and eventual opening. We previously identified MISREGULATED IN DARK9 (MIDA9) as a phytochrome interacting factor (PIF)-repressed gene in the dark necessary for hook development during etiolated growth. MIDA9 encodes the type 2C phosphatase PP2C.D1, and pp2c-d1/mida9 mutants exhibit open hooks in the dark. Recent evidence has described that PP2C.D1 and other PP2C.D members negatively regulate SMALL AUXIN UP RNA (SAUR)-mediated cell elongation. However, the fundamental question of the timing of PP2C.D1 action (and possibly other members of the PP2C.D family) during hook development remains to be addressed. Here, we show that PP2C.D1 is required immediately after germination to form the hook. pp2c.d1/mida9 shows reduced cell expansion in the outer layer of the hook and, therefore, does not establish the differential cell growth necessary for hook formation, indicating that PP2C.D1 is necessary to promote cell elongation during this early stage. Additionally, genetic analyses of single and high order mutants in PP2C.D1, PP2C.D2, and PP2C.D5 demonstrate that the three PP2C.Ds act collectively and sequentially during etiolation: whereas PP2C.D1 dominates hook formation, PP2C.D2 is necessary during the maintenance phase, and PP2C.D5 acts to prevent opening during the third phase together with PP2C.D1 and PP2C.D2. Finally, we uncover a possible connection of PP2C.D1 levels with ethylene physiology, which could help optimize hook formation during post-germinative growth in the dark.ca
dc.format.extent12 p.ca
dc.language.isoengca
dc.publisherFrontiers Mediaca
dc.relation.ispartofFrontiers in Plant Scienceca
dc.rights© L'autor/aca
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherSkotomorphogenesisca
dc.subject.otherEtiolationca
dc.subject.otherHookca
dc.subject.otherMIDA9ca
dc.subject.otherPP2C.D phosphatasesca
dc.subject.otherPhytochrome interacting factor PIFca
dc.subject.otherEthyleneca
dc.subject.otherEtilèca
dc.subject.otherGerminacióca
dc.titleThe sequential action of MIDA9/PP2C.D1, PP2C.D2, and PP2C.D5 is necessary to form and maintain the hook after germination in the darkca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc5ca
dc.identifier.doihttps://doi.org/10.3389/fpls.2021.636098ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/Marie Curie/IRG PIRG06-GA-2009-256420ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIU i FEDER/PN I+D/BIO2009-07675ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIU i FEDER/PN I+D/BIO2015-68460-Pca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIU i FEDER/PN I+D/PGC2018-099987-B-I00ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/SUR del DEC/SGR/2009-SGR-206ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/SUR del DEC/SGR/2017-SGR-718ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Centro de Excelencia Severo Ochoa 2016–2019 Program/SEV-2015-0533ca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca


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