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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorFaria, Melissa
dc.contributor.authorPrats, Eva
dc.contributor.authorBellot, Marina
dc.contributor.authorGómez Canela, Cristian
dc.contributor.authorRaldúa, Demetrio
dc.date.accessioned2024-05-23T19:50:37Z
dc.date.available2024-05-23T19:50:37Z
dc.date.issued2021-05-25
dc.identifier.issn2305-6304ca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/4076
dc.description.abstractThis study examines the effects of acute pharmacological modulation of the serotonergic system over zebrafish larvae’s cognitive, basic, and defense locomotor behaviors, using a medium to high throughput screening assay. Furthermore, the relationship between behavior, enzyme activity related to neurotransmitter metabolism, neurotransmitter levels, and gene expression was also determined. Modulation of larvae serotonergic system was accomplished by 24 h exposure to single and opposite pharmacodynamics co-exposure to three model psychopharmaceuticals with antagonistic and agonistic serotonin signaling properties: 2.5 mM 4-Chloro-DL-phenylalanine (PCPA) and 5 µM deprenyl and 0.5 µM fluoxetine, respectively. Similar behavioral outcome was observed for deprenyl and fluoxetine, which was reflected as hypolocomotion, decrease in larvae defensive responses, and cognitive impairment. Contrarily, PCPA induced hyperlocomotion and increase in larvae escape response. Deprenyl exposure effects were more pronounced at a lower level of organization than fluoxetine, with complete inhibition of monoamine oxidase (MAO) activity, dramatic increase of 5-HT and dopamine (DA) levels, and downregulation of serotonin synthesis and transporter genes. PCPA showed mainly effects over serotonin and dopamine’s main degradation metabolites. Finally, co-exposure between agonistic and antagonist serotonin signaling drugs reviled full recovery of zebrafish impaired locomotor and defense responses, 5-HT synthesis gene expression, and partial recovery of 5-HT levels. The findings of this study suggest that zebrafish larvae can be highly sensitive and a useful vertebrate model for short-term exposure to serotonin signaling changesca
dc.format.extent7ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofToxicsca
dc.rights© L'autor/aca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherPeix zebraca
dc.subject.otherPeix zebra--Hàbits i conductaca
dc.subject.otherSerotoninaca
dc.subject.otherNeurotransmissorsca
dc.subject.otherModulationca
dc.titlePharmacological modulation of serotonin levels in zebrafish larvae: lessons for identifying environmental neurotoxicants targeting the serotonergic systemca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc615ca
dc.identifier.doihttps://doi.org/10.3390/toxics9060118ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/IDAEA-CSIC/CEX2018-000794-Sca
dc.relation.projectIDinfo:eu-repo/grantAgreement/SUR del DEC/SGR/2017 SGR_902ca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca


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