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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorArtigues Cladera, Margalida
dc.contributor.authorGilabert-Porres, Joan
dc.contributor.authorTexidó Bartes, Robert
dc.contributor.authorBorrós i Gómez, Salvador
dc.contributor.authorAbellà, Jordi (Abellà i Iglesias)
dc.contributor.authorColominas Fuster, Sergi
dc.date.accessioned2024-02-21T18:34:13Z
dc.date.available2024-02-21T18:34:13Z
dc.date.issued2021-06-15
dc.identifier.issn1424-8220ca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/3944
dc.description.abstractBioanalytical methods, in particular electrochemical biosensors, are increasingly used in different industrial sectors due to their simplicity, low cost, and fast response. However, to be able to reliably use this type of device, it is necessary to undertake in-depth evaluation of their fundamental analytical parameters. In this work, analytical parameters of an amperometric biosensor based on covalent immobilization of glucose oxidase (GOx) were evaluated. GOx was immobilized using plasma-grafted pentafluorophenyl methacrylate (pgPFM) as an anchor onto a tailored HEMA-co-EGDA hydrogel that coats a titanium dioxide nanotubes array (TiO2NTAs). Finally, chitosan was used to protect the enzyme molecules. The biosensor offered outstanding analytical parameters: repeatability (RSD = 1.7%), reproducibility (RSD = 1.3%), accuracy (deviation = 4.8%), and robustness (RSD = 2.4%). In addition, the Ti/TiO2NTAs/ppHEMA-co-EGDA/pgPFM/GOx/Chitosan biosensor showed good long-term stability; after 20 days, it retained 89% of its initial sensitivity. Finally, glucose concentrations of different food samples were measured and compared using an official standard method (HPLC). Deviation was lower than 10% in all measured samples. Therefore, the developed biosensor can be considered to be a reliable analytical tool for quantification measurements.ca
dc.format.extent15 p.ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofSensorsca
dc.rights© L'autor/aca
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherBioanalytical methodsca
dc.subject.otherElectrochemical biosensorca
dc.subject.otherHydroxyethyl methacrylate (HEMA)ca
dc.subject.otherPentafluorophenyl methacrylate (PFM)ca
dc.subject.otherGlucose oxidase (GOx)ca
dc.titleAnalytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization techniqueca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc6ca
dc.identifier.doihttps://doi.org/10.3390/s21124185ca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca


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