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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorGuarro, Mònica
dc.contributor.authorSuñer, Francisca
dc.contributor.authorLecina i Veciana, Martí
dc.contributor.authorBorrós i Gómez, Salvador
dc.contributor.authorFornaguera Puigvert, Cristina
dc.date.accessioned2024-02-08T13:26:08Z
dc.date.issued2022-07-31
dc.identifier.issn1873-4367ca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/3902
dc.description.abstractDespite the great knowledge achieved in the field of extracellular vesicles (EVs), the short lifetime of EVs liquid formulation still hampers the transfer of EVs technology to clinical applications. In this context, freeze-dried EVs would be advantageous thanks to the enhanced stability of solid formulations. Although some previous attempts have already been reported, the efficiency of EVs lyophilization methodologies used remains insufficient, and the characterization of the resulting EVs is still incomplete. The current work aims to describe an alternative and easy-to-be-applied methodology for EVs lyophilization. The use of sucrose as lyoprotectant at 8.5%wt improved the cryopreservation efficiency. After the subsequent cycles of freeze-drying, properties such as size, morphology, purity, EVs specific markers, biocompatibility and the maintenance of their functionality were confirmed in freeze-dried EVs samples. To sum up, we have designed a methodology for the lyophilization of extracellular vesicles that enables the preservation of the physicochemical properties and functionality of EVs.ca
dc.format.extent22 p.ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofColloids and Surfaces B: Biointerfacesca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights© Elsevier*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.otherFreeze-dryingca
dc.subject.otherExtracellular Vesiclesca
dc.subject.otherSucroseca
dc.subject.otherPhysicochemical Characterizationca
dc.subject.otherEVs Preservationca
dc.titleEfficient extracellular vesicles freeze-dry method for direct formulations preparation and useca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/embargoedAccess
dc.date.embargoEnd2024-07-30T02:00:00Z
dc.embargo.terms24 mesosca
dc.subject.udc576ca
dc.identifier.doihttps://doi.org/10.1016/j.colsurfb.2022.112745ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO i FEDER/PN I+D/SAF2015–64927-C2–2-Rca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO i FEDER/PN I+D/RTI2018–094734-B-C22ca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/
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