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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorWatson, Joseph
dc.contributor.authorAich, Samya
dc.contributor.authorOller Salvia, Benjamí
dc.contributor.authorDrabek, Andrew A.
dc.contributor.authorBlacklow, Stephen
dc.contributor.authorChin, Jason
dc.contributor.authorDerivery, Emmanuel
dc.date.accessioned2023-12-10T22:24:31Z
dc.date.available2023-12-10T22:24:31Z
dc.date.issued2021-01-08
dc.identifier.issn1540-8140ca
dc.identifier.urihttp://hdl.handle.net/20.500.14342/3639
dc.description.abstractProtein micropatterning allows proteins to be precisely deposited onto a substrate of choice and is now routinely used in cell biology and in vitro reconstitution. However, drawbacks of current technology are that micropatterning efficiency can be variable between proteins and that proteins may lose activity on the micropatterns. Here, we describe a general method to enable micropatterning of virtually any protein at high specificity and homogeneity while maintaining its activity. Our method is based on an anchor that micropatterns well, fibrinogen, which we functionalized to bind to common purification tags. This enhances micropatterning on various substrates, facilitates multiplexed micropatterning, and dramatically improves the on-pattern activity of fragile proteins like molecular motors. Furthermore, it enhances the micropatterning of hard-to-micropattern cells. Last, this method enables subcellular micropatterning, whereby complex micropatterns simultaneously control cell shape and the distribution of transmembrane receptors within that cell. Altogether, these results open new avenues for cell biology.ca
dc.format.extent28 p.ca
dc.language.isoengca
dc.publisherRockefeller University Pressca
dc.relation.ispartofJournal of Cell Biologyca
dc.rights© L'autor/aca
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.otherAdherènciaca
dc.subject.otherTecnologiaca
dc.titleHigh-efficacy subcellular micropatterning of proteins using fibrinogen anchorsca
dc.typeinfo:eu-repo/semantics/articleca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc62ca
dc.identifier.doihttps://dx.doi.org/10.1083/jcb.202009063ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/Medical Research Council/MC_UP_1201/13ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/Human Frontier Science Program/Career Development Award CDA00034/2017-Cca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca


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Mostra el registre parcial de l'element

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