Visualitzant Articles publicats en revistes per autor/a "Lecina i Veciana, Martí"
Ara mostrant els elements 1-6 de 6
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A simplified implementation of the stationary liquid mass balance method for on‐line OUR monitoring in animal cell cultures
Lecina i Veciana, Martí; Fontova i Sosa, Andreu Francesc; López Repullo, Jonatan; Martínez Monge, Iván; Comas Sánchez, Pere; Bragós Bardia, Ramon; Cairó, Jordi Joan (Journal of Chemical Technology and Biotechnology. Vol.93, n.6 (2018), p.1757-1766, 2018-05)BACKGROUND Compared with other methods, the stationary liquid mass balance method for oxygen uptake rate (OUR) determination offers advantages in terms of estimation accuracy and reduction of stress. ... -
Development of a non-viral platform for rapid virus-like particle production in Sf9 cells
Puente Massaguer, Eduard; Gòdia, Francesc; Lecina i Veciana, Martí (Journal of Biotechnology, 2020-07-11)Insect cells have shown a high versatility to produce multiple recombinant products. The ease of culture, low contamination risk with human pathogens and high expression capacity makes an attractive ... -
Efficient extracellular vesicles freeze-dry method for direct formulations preparation and use
Guarro, Mònica; Suñer, Francisca; Lecina i Veciana, Martí; Borrós i Gómez, Salvador; Fornaguera Puigvert, Cristina (Colloids and Surfaces B: Biointerfaces, 2022-07-31)Despite the great knowledge achieved in the field of extracellular vesicles (EVs), the short lifetime of EVs liquid formulation still hampers the transfer of EVs technology to clinical applications. In ... -
Metabolic flux balance analysis during lactate and glucose concomitant consumption in HEK293 cell cultures
Martínez-Monge, Iván; Albiol, Joan; Lecina i Veciana, Martí; Liste Calleja, Leticia; Miret, Joan; Solà, Carles; Cairó, Jordi J. (Biotechnology and Bioengineering, 2018-10-26)At early stages of the exponential growth phase in HEK293 cell cultures, the tricarboxylic acid cycle is unable to process all the amount of NADH generated in the glycolysis pathway, being lactate the ... -
Nanoscale characterization coupled to multi-parametric optimization of Hi5 cell transient gene expression
Puente-Massaguer, Eduard; Lecina i Veciana, Martí; Gòdia, Francesc (Applied Microbiology and Biotechnology, 2018-10-13)Polyethylenimine (PEI)-based transient gene expression (TGE) is nowadays a well-established methodology for rapid protein production in mammalian cells, but it has been used to a much lower extent in ... -
PEI-mediated transient transfection of high five cells at bioreactor scale for HIV-1 VLP production
Lecina i Veciana, Martí; Puente-Massaguer, Eduard; Strobl, Florian; Grabherr, Reingard; Striedner, Gerald; Gòdia, Francesc (Nanomaterials. Vol.10, n.8 (2020), 1580, 2020-08)High Five cells are an excellent host for the production of virus-like particles (VLPs) with the baculovirus expression vector system (BEVS). However, the concurrent production of high titers of baculovirus ...