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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorGarcía Granada, Andrés-Amador
dc.contributor.authorBahmaee, Hossein
dc.contributor.authorOwen, Robert
dc.contributor.authorBoyle, Liam
dc.contributor.authorPerrault, Cecile M.
dc.contributor.authorReilly, Gwendolen C.
dc.contributor.authorClaeyssens, Frederik
dc.date.accessioned2021-07-29T06:08:42Z
dc.date.accessioned2023-07-13T05:45:37Z
dc.date.available2021-07-29T06:08:42Z
dc.date.available2023-07-13T05:45:37Z
dc.date.issued2020-09
dc.identifier.urihttp://hdl.handle.net/20.500.14342/1103
dc.description.abstractMicrofluidic-based tissue-on-a-chip devices have generated significant research interest for biomedical applications, such as pharmaceutical development, as they can be used for small volume, high throughput studies on the effects of therapeutics on tissue-mimics. Tissue-on-a-chip devices are evolving from basic 2D cell cultures incorporated into microfluidic devices to complex 3D approaches, with modern designs aimed at recapitulating the dynamic and mechanical environment of the native tissue. Thus far, most tissue-on-a-chip research has concentrated on organs involved with drug uptake, metabolism and removal (e.g., lung, skin, liver, and kidney); however, models of the drug metabolite target organs will be essential to provide information on therapeutic efficacy. Here, we develop an osteogenesis-on-a-chip device that comprises a 3D environment and fluid shear stresses, both important features of bone. This inexpensive, easy-to-fabricate system based on a polymerized High Internal Phase Emulsion (polyHIPE) supports proliferation, differentiation and extracellular matrix production of human embryonic stem cell-derived mesenchymal progenitor cells (hES-MPs) over extended time periods (up to 21 days). Cells respond positively to both chemical and mechanical stimulation of osteogenesis, with an intermittent flow profile containing rest periods strongly promoting differentiation and matrix formation in comparison to static and continuous flow. Flow and shear stresses were modeled using computational fluid dynamics. Primary cilia were detectable on cells within the device channels demonstrating that this mechanosensory organelle is present in the complex 3D culture environment. In summary, this device aids the development of ‘next-generation’ tools for investigating novel therapeutics for bone in comparison with standard laboratory and animal testing.eng
dc.format.extent17 p.cat
dc.language.isoengcat
dc.publisherFrontiers Mediacat
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology. Vol.8 (2018), 557111cat
dc.rightsAttribution 4.0 International
dc.rights© L'autor/a
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherÒrgans (Anatomia)cat
dc.subject.otherEnginyeria de teixitscat
dc.subject.otherBiologia computacionalcat
dc.subject.otherOrgan-on-a-chipcat
dc.subject.otherMechanotransductioncat
dc.subject.otherPolyHIPEcat
dc.subject.otherAdditive manufacturecat
dc.subject.otherBioreactorcat
dc.subject.otherComputational fluid dynamicscat
dc.subject.otherTissue engineeringcat
dc.titleDesign and evaluation of an osteogenesis-on-a-chip microfluidic device incorporating 3D cell culturecat
dc.typeinfo:eu-repo/semantics/articlecat
dc.typeinfo:eu-repo/semantics/publishedVersioncat
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapcat
dc.subject.udc61
dc.subject.udc62
dc.identifier.doihttps://doi.org/10.3389/fbioe.2020.557111cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/EPSRC/Grant No. EP/N509735/1cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/EPSRC/Grant No. EP/L505055/1cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/BBSRC/Grant No. BB/F016840/1cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/EPSRC Henry Royce Institute funding/Grant No. EP/P02470X/1cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/EPSRC/Grant No. EP/I007695/1cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/MRC/Grant No. MR/L012669/1cat


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Attribution 4.0 International
Excepte que s'indiqui una altra cosa, la llicència de l'ítem es descriu com http://creativecommons.org/licenses/by/4.0/
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