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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorAgut Bonsfills, Montserrat
dc.contributor.authorNonell, Santi
dc.contributor.authorDelcanale, Pietro
dc.contributor.authorPennacchietti, Francesca
dc.contributor.authorMaestrini, Giulio
dc.contributor.authorRodríguez Amigo, Beatriz
dc.contributor.authorBianchini, Paolo
dc.contributor.authorDiaspro, Alberto
dc.contributor.authorIagatti, Alessandro
dc.contributor.authorPatrizi, Barbara
dc.contributor.authorFoggi, Paolo
dc.contributor.authorAbbruzzetti, Stefania
dc.contributor.authorViappiani, Cristiano
dc.date.accessioned2020-07-01T16:12:55Z
dc.date.accessioned2023-07-13T05:44:13Z
dc.date.available2020-07-01T16:12:55Z
dc.date.available2023-07-13T05:44:13Z
dc.date.issued2015-10
dc.identifier.urihttp://hdl.handle.net/20.500.14342/1024
dc.description.abstractAntibacterial treatments based on photosensitized production of reactive oxygen species is a promising approach to address local microbial infections. Given the small size of bacterial cells, identification of the sites of binding of the photosensitizing molecules is a difficult issue to address with conventional microscopy. We show that the excited state properties of the naturally occurring photosensitizer hypericin can be exploited to perform STED microscopy on bacteria incubated with the complex between hypericin and apomyoglobin, a self-assembled nanostructure that confers very good bioavailability to the photosensitizer. Hypericin fluorescence is mostly localized at the bacterial wall and accumulates at the polar regions of the cell and at sites of cell wall growth. While these features are shared by Gram-negative and Gram-positive bacteria, only the latter are effectively photoinactivated by light exposure.eng
dc.format.extent9 p.
dc.language.isoeng
dc.publisherNature Research
dc.relation.ispartofScientific Reports. Vol.5, 15564 (2015)
dc.rightsAttribution 4.0 International
dc.rights© L'autor/a
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherBiofísica
dc.subject.otherFotosensibilització (Biologia)
dc.subject.otherAntimicrobials
dc.subject.otherBiophysics
dc.titleSubdiffraction localization of a nanostructured photosensitizer in bacterial cells
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscap
dc.subject.udc577
dc.identifier.doihttps://doi.org/10.1038/srep15564


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Mostra el registre parcial de l'element

Attribution 4.0 International
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