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dc.contributorUniversitat Ramon Llull. IQS
dc.contributor.authorSemino, Carlos
dc.contributor.authorAloy Reverté, Caterina
dc.contributor.authorMoreno Amador, José Luis
dc.contributor.authorTéllez i Besolí, Noèlia
dc.contributor.authorMarin, Sandra
dc.contributor.authorNacher, Montserrat
dc.contributor.authorMontanya Mias, Eduard
dc.date.accessioned2020-07-03T11:54:25Z
dc.date.accessioned2023-07-13T05:44:03Z
dc.date.available2020-07-03T11:54:25Z
dc.date.available2023-07-13T05:44:03Z
dc.date.issued2018-01
dc.identifier.urihttp://hdl.handle.net/20.500.14342/1015
dc.description.abstractBackground β-cells undergo an epithelial to mesenchymal transition (EMT) when expanded in monolayer culture and give rise to highly proliferative mesenchymal cells that retain the potential to re-differentiate into insulin-producing cells. Objective To investigate whether EMT takes place in the endocrine non-β cells of human islets. Methodology Human islets isolated from 12 multiorgan donors were dissociated into single cells, purified by magnetic cell sorting, and cultured in monolayer. Results Co-expression of insulin and the mesenchymal marker vimentin was identified within the first passage (p1) and increased subsequently (insulin+vimentin+ 7.2±6% at p1; 43±15% at p4). The endocrine non-β-cells did also co-express vimentin (glucagon+vimentin+ 59±1.5% and 93±6%, somatostatin+vimentin+ 16±9.4% and 90±10% at p1 and p4 respectively; PP+vimentin+ 74±14% at p1; 88±12% at p2). The percentage of cells expressing only endocrine markers was progressively reduced (0.6±0.2% insulin+, 0.2±0.1% glucagon+, and 0.3±0.2% somatostatin+ cells at p4, and 0.7±0.3% PP+ cells at p2. Changes in gene expression were also indicated of EMT, with reduced expression of endocrine markers and the epithelial marker CDH-1 (p<0.01), and increased expression of mesenchymal markers (CDH-2, SNAI2, ZEB1, ZEB2, VIM, NT5E and ACTA2; p<0.05). Treatment with the EMT inhibitor A83-01 significantly reduced the percentage of co-expressing cells and preserved the expression of endocrine markers. Conclusions In adult human islets, all four endocrine islet cell types undergo EMT when islet cells are expanded in monolayer conditions. The presence of EMT in all islet endocrine cells could be relevant to design of strategies aiming to re-differentiate the expanded islet cells towards a β-cell phenotype.eng
dc.format.extent18 p.cat
dc.language.isoengcat
dc.publisherPLoS (Public Library of Science)cat
dc.relation.ispartofPLoS One. Vol. 13, n.1 (2018), p. e0191104cat
dc.rights© L'autor/a
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceRECERCAT (Dipòsit de la Recerca de Catalunya)
dc.subject.otherCèl·lules epitelialscat
dc.subject.otherExpressió gènicacat
dc.subject.otherInsulinacat
dc.subject.otherMesenchymal transitioncat
dc.subject.otherMesenchymal cellscat
dc.subject.otherInsulin-producing cellscat
dc.titleEpithelial to mesenchymal transition in human endocrine islet cellscat
dc.typeinfo:eu-repo/semantics/articlecat
dc.typeinfo:eu-repo/semantics/publishedVersioncat
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapcat
dc.subject.udc576
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0191104cat
dc.relation.projectIDinfo:eu-repo/grantAgreement/Fundació La Marató TV3/PR084/12cat


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