Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy
Author
Nonell, Santi
Ruiz González, Rubén
Torra, Joaquim
Rodríguez-Pulido, Alberto
Cortajarena, Aitziber L.
Flors, Cristina
Other authors
Universitat Ramon Llull. IQS
Publication date
2016-06Abstract
Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the
photostability of miniSOG mutants and their ability to photooxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.
Document Type
Article
Published version
Language
English
Subject (CDU)
54 - Chemistry. Crystallography. Mineralogy
Keywords
Flavoproteïnes
Microscòpia
Fotosensibilització (Biologia)
Photosensitizing flavoproteins
Correlative microscopy
Photo-oxidation
Pages
4 p.
Publisher
Royal Society of Chemistry
Is part of
Chemical Communications. Vol.52, n.54 (2016), p.8405-8408
Grant agreement number
info:eu-repo/grantAgreement/MINECO/PN I+D/RyC2011-07637
info:eu-repo/grantAgreement/MINECO/PN I+D/MAT2012-34487
info:eu-repo/grantAgreement/MINECO/PN I+D/BIO2012-34835
info:eu-repo/grantAgreement/MINECO/PN I+D/CTQ2013-48767-C3-1-R
info:eu-repo/grantAgreement/MINECO/PN I+D/CTQ2015-71896-REDT
info:eu-repo/grantAgreement/MINECO/PN I+D/MAT2015-66605-P
info:eu-repo/grantAgreement/EC/FP7/FP7-PEOPLE-2010-IRG-246688
info:eu-repo/grantAgreement/EC/FP7FP7-PEOPLE-2011-CIG-303620/
info:eu-repo/grantAgreement/EC/FP7/FP7-PEOPLE-2011-COFUND-291803
This item appears in the following Collection(s)
Rights
© The Royal Society of Chemistry
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/