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    • Application of flow cytometry analysis to elucidate the impact of scale-down conditions in Escherichia coli cultivations 

      Turon Barrera, Xavier; Marba-Ardebol, Anna M; Neubauer, Peter B.; Junne, Stefan (Afinidad. Vol.73, n.573 (2016), p.7-15, 2016-01)
      In homogeneities appear in large-scale fed-batch bioprocesses; especially when the distribution of the feeding solution and of the oxygen is uneven. In order to study the consequences of these heterogenic ...

    • Efficient extracellular vesicles freeze-dry method for direct formulations preparation and use 

      Guarro, Mònica; Suñer, Francisca; Lecina i Veciana, Martí; Borrós i Gómez, Salvador; Fornaguera Puigvert, Cristina (Colloids and Surfaces B: Biointerfaces, 2022-07-31)
      Despite the great knowledge achieved in the field of extracellular vesicles (EVs), the short lifetime of EVs liquid formulation still hampers the transfer of EVs technology to clinical applications. In ...

    • Epithelial to mesenchymal transition in human endocrine islet cells 

      Semino, Carlos; Aloy Reverté, Caterina; Moreno Amador, José Luis; Téllez i Besolí, Noèlia; Marin, Sandra; Nacher, Montserrat; Montanya Mias, Eduard (PLoS One. Vol. 13, n.1 (2018), p. e0191104, 2018-01)
      Background β-cells undergo an epithelial to mesenchymal transition (EMT) when expanded in monolayer culture and give rise to highly proliferative mesenchymal cells that retain the potential to ...

    • Monitoring Tools for the Development of High Cell Density Culture Strategies 

      Lecina i Veciana, Martí; Comas, Pere; Martínez Monge, Iván; Cairó, Jordi J. (Cell Culture Engineering and Technology. Cell Engineering, 2022-02-20)
      Biopharmaceuticals market has been constantly increasing during the last years, what is transforming the manufacturing industry of biomolecules. The efforts have been put into better understanding how ...

    • Simple generation of human induced pluripotent stem cells using poly-β-amino esters as the non-viral gene delivery system 

      Borrós i Gómez, Salvador; Ramos Pérez, Víctor; Gutiérrez, Jordán; Izpisúa Belmonte, J. C.; Montserrat, Núria; Garreta, Elena; González, Federico; Eguizábal, Cristina (Journal of Biological Chemistry. Vol.286,n.14 (2011),p.12417-12428, 2011-04)
      Reprogramming of somatic cells to induced pluripotent stem (iPS) cells can be achieved by the delivery of a combination of transcription factors, including Oct4, Sox2, Klf4, and c-Myc. Retroviral and ...