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Enzymatic Hydrolysis of Human Milk Oligosaccharides. The Molecular Mechanism of Bifidobacterium Bifidum Lacto-N-biosidase
(American Chemical Society, 2022)
Bifidobacterium bifidum lacto-N-biosidase (LnbB) is a critical enzyme for the degradation of human milk oligosaccharides in the gut microbiota of breast-fed infants. Guided by recent crystal structures, ...
Structural-functional analysis reveals a specific domain organization in family GH20 hexosaminidases
(Public Library of Science (PLoS), 2015-05)
Hexosaminidases are involved in important biological processes catalyzing the hydrolysis of N-acetyl-hexosaminyl residues in glycosaminoglycans and glycoconjugates. The GH20 enzymes present diverse ...
Structure-function features of a mycoplasma glycolipid synthase derived from structural data integration, molecular simulations, and mutational analysis
(Public Library of Science (PLoS), 2013-12)
Glycoglycerolipids are structural components of mycoplasma membranes with a fundamental role in membrane properties and stability. Their biosynthesis is mediated by glycosyltransferases (GT) that catalyze ...
Formation of a Covalent Adduct in Retaining β-Kdo Glycosyl-Transferase WbbB via Substrate-Mediated Proton Relay
(Wiley, 2024-07-22)
The GT99 domain of the membrane-anchored WbbB glycosyltransferase (WbbBGT99) catalyzes the transfer of 3-deoxy-D-manno-oct-2-acid (β-Kdo) to an O-antigen saccharide acceptor with retention of stereochemistry. ...
Novel α-mannose-functionalized poly(β-amino ester) nanoparticles as mRNA vaccines with increased antigen presenting cell selectivity in the spleen
(RSC, 2023-06-14)
mRNA vaccination has emerged as a prominent therapy for the future of medicine. Despite the colossal advance in this technology and worldwide efficacy proof (ca. COVID vaccines), mRNA carriers still ...
Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis
(MDPI, 2021-03-21)
The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks. However, HMO synthesis is still challenging and only two HMOs have been ...
Bioprocess Strategies for Vitamin B12 Production by Microbial Fermentation and Its Market Applications
(MDPI, 2022)
Vitamin B12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. For this ...
Integrative structure determination reveals functional global flexibility for an ultra-multimodular arabinanase
(Nature Research, 2022)
AbnA is an extracellular GH43 α-L-arabinanase from Geobacillus stearothermophilus, a key bacterial enzyme in the degradation and utilization of arabinan. We present herein its full-length crystal ...
Chloroplast engineering of the green microalgae Chlamydomonas reinhardtii for the production of HAA, the lipid moiety of rhamnolipid biosurfactants
(Elsevier, 2023-04-11)
Hydroxyalkanoyloxyalkanoates (HAA) are lipidic surfactants with a number of potential applications, but more remarkably, they are the biosynthetic precursors of rhamnolipids (RL), which are preferred ...
Characterization of a Glycolipid Synthase Producing α-Galactosylceramide in Bacteroides fragilis
(2022)
Glycolipids are complex molecules involved in important cellular processes. Among them, the glycosphingolipid α-galactosylceramide has proven to be of interest in biomedicine for its immunostimulatory ...