Browsing by Author "Lecina i Veciana, Martí"
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A simplified implementation of the stationary liquid mass balance method for on‐line OUR monitoring in animal cell cultures
Lecina i Veciana, Martí; Fontova i Sosa, Andreu Francesc; López Repullo, Jonatan; Martínez-Monge, Iván; Comas Sánchez, Pere; Bragós Bardia, Ramon; Cairó, Jordi Joan (Journal of Chemical Technology and Biotechnology. Vol.93, n.6 (2018), p.1757-1766, 2018-05)BACKGROUND Compared with other methods, the stationary liquid mass balance method for oxygen uptake rate (OUR) determination offers advantages in terms of estimation accuracy and reduction of stress. ... -
Development of a non-viral platform for rapid virus-like particle production in Sf9 cells
Puente-Massaguer, Eduard; Gòdia, Francesc; Lecina i Veciana, Martí (Journal of Biotechnology, 2020-07-11)Insect cells have shown a high versatility to produce multiple recombinant products. The ease of culture, low contamination risk with human pathogens and high expression capacity makes an attractive ... -
Efficient extracellular vesicles freeze-dry method for direct formulations preparation and use
Guarro, Mònica; Suñer, Francisca; Lecina i Veciana, Martí; Borrós i Gómez, Salvador; Fornaguera Puigvert, Cristina (Colloids and Surfaces B: Biointerfaces, 2022-07-31)Despite the great knowledge achieved in the field of extracellular vesicles (EVs), the short lifetime of EVs liquid formulation still hampers the transfer of EVs technology to clinical applications. In ... -
Identification of critical process parameters for expansion of clinical grade human Wharton's jelly-derived mesenchymal stromal cells in stirred-tank bioreactors
López-Fernández, Alba; Garcia-Gragera, Víctor; Lecina i Veciana, Martí; Vives, Joaquim (Biotechnology Journal. 2024;19:2300381, 2024-02-25)Cell therapies based on multipotent mesenchymal stromal cells (MSCs) are traditionally produced using 2D culture systems and platelet lysate- or serum-containing media (SCM). Although cost-effective for ... -
Metabolic flux balance analysis during lactate and glucose concomitant consumption in HEK293 cell cultures
Martínez-Monge, Iván; Albiol, Joan; Lecina i Veciana, Martí; Liste Calleja, Leticia; Miret, Joan; Solà, Carles; Cairó, Jordi Joan (Biotechnology and Bioengineering, 2018-10-26)At early stages of the exponential growth phase in HEK293 cell cultures, the tricarboxylic acid cycle is unable to process all the amount of NADH generated in the glycolysis pathway, being lactate the ... -
Monitoring Tools for the Development of High Cell Density Culture Strategies
Lecina i Veciana, Martí; Comas Sánchez, Pere; Martínez-Monge, Iván; Cairó, Jordi Joan (Cell Culture Engineering and Technology. Cell Engineering, 2022-02-20)Biopharmaceuticals market has been constantly increasing during the last years, what is transforming the manufacturing industry of biomolecules. The efforts have been put into better understanding how ... -
Nanoscale characterization coupled to multi-parametric optimization of Hi5 cell transient gene expression
Puente-Massaguer, Eduard; Lecina i Veciana, Martí; Gòdia, Francesc (Applied Microbiology and Biotechnology, 2018-10-13)Polyethylenimine (PEI)-based transient gene expression (TGE) is nowadays a well-established methodology for rapid protein production in mammalian cells, but it has been used to a much lower extent in ... -
PEI-mediated transient transfection of high five cells at bioreactor scale for HIV-1 VLP production
Lecina i Veciana, Martí; Puente-Massaguer, Eduard; Strobl, Florian; Grabherr, Reingard; Striedner, Gerald; Gòdia, Francesc (Nanomaterials. Vol.10, n.8 (2020), 1580, 2020-08)High Five cells are an excellent host for the production of virus-like particles (VLPs) with the baculovirus expression vector system (BEVS). However, the concurrent production of high titers of baculovirus ... -
The Effect of the Expression of the Antiapoptotic BHRF1 Gene on the Metabolic Behavior of a Hybridoma Cell Line
Martínez-Monge, Iván; Comas Sánchez, Pere; Catalán-Tatjer, David; Prat, Jordi; Casablancas, Antoni; Paredes, Carlos J.; Lecina i Veciana, Martí; Cairó, Jordi Joan (Applied Sciences, 2021-07-06)One of the most important limitations of mammalian cells-based bioprocesses, and particularly hybridoma cell lines, is the accelerated metabolism related to glucose and glutamine consumption. The high ...