Browsing by Author "Puente-Massaguer, Eduard"
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Development of a non-viral platform for rapid virus-like particle production in Sf9 cells
Puente-Massaguer, Eduard; Gòdia, Francesc; Lecina i Veciana, Martí (Journal of Biotechnology, 2020-07-11)Insect cells have shown a high versatility to produce multiple recombinant products. The ease of culture, low contamination risk with human pathogens and high expression capacity makes an attractive ... -
Nanoscale characterization coupled to multi-parametric optimization of Hi5 cell transient gene expression
Puente-Massaguer, Eduard; Lecina i Veciana, Martí; Gòdia, Francesc (Applied Microbiology and Biotechnology, 2018-10-13)Polyethylenimine (PEI)-based transient gene expression (TGE) is nowadays a well-established methodology for rapid protein production in mammalian cells, but it has been used to a much lower extent in ... -
PEI-mediated transient transfection of high five cells at bioreactor scale for HIV-1 VLP production
Lecina i Veciana, Martí; Puente-Massaguer, Eduard; Strobl, Florian; Grabherr, Reingard; Striedner, Gerald; Gòdia, Francesc (Nanomaterials. Vol.10, n.8 (2020), 1580, 2020-08)High Five cells are an excellent host for the production of virus-like particles (VLPs) with the baculovirus expression vector system (BEVS). However, the concurrent production of high titers of baculovirus ...