Browsing by Author "Planas, Antoni (Planas Sauter)"
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Bioprocess Strategies for Vitamin B12 Production by Microbial Fermentation and Its Market Applications
Calvillo, Alvaro; Pellicer Moya, Maria Teresa; Carnicer, Marc; Planas, Antoni (Planas Sauter) (Bioengineering 2022, 9(8), 365, 2022)Vitamin B12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. For this ... -
Characterization of a Glycolipid Synthase Producing α-Galactosylceramide in Bacteroides fragilis
Caballé, Marc; Faijes, Magda; Planas, Antoni (Planas Sauter) (International Journal of Molecular Sciences 2022, 23(22), 13975, 2022)Glycolipids are complex molecules involved in important cellular processes. Among them, the glycosphingolipid α-galactosylceramide has proven to be of interest in biomedicine for its immunostimulatory ... -
Chloroplast engineering of the green microalgae Chlamydomonas reinhardtii for the production of HAA, the lipid moiety of rhamnolipid biosurfactants
Miró-Vinyals, Bernat; Artigues Cladera, Margalida; Wostrikoff, Katia; Monte, Elena; Broto Puig, Francesc; Leivar Rico, Pablo; Planas, Antoni (Planas Sauter) (New Biotechnology, 2023-04-11)Hydroxyalkanoyloxyalkanoates (HAA) are lipidic surfactants with a number of potential applications, but more remarkably, they are the biosynthetic precursors of rhamnolipids (RL), which are preferred ... -
Control of Substrate Conformation by Hydrogen Bonding in a Retaining β-Endoglycosidase
Nin-Hill, Alba; Ardevol, Albert; Biarnés Fontal, Xevi; Planas, Antoni (Planas Sauter); Rovira, Carme (Chemistry - A European Journal 2023, 29(70), e202302555, 2023-12-14)Bacterial β-glycosidases are hydrolytic enzymes that depolymerize polysaccharides such as β-cellulose, β-glucans and β-xylans from different sources, offering diverse biomedical and industrial uses. It ... -
Developing a single-stage continuous process strategy for vitamin B12 production with Propionibacterium freudenreichii
Calvillo, Álvaro; Pellicer Moya, Maria Teresa; Carnicer, Marc; Planas, Antoni (Planas Sauter) (Microbial Cell Factories 2023, 22, 2023)Background: Vitamin B12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. ... -
Enzymatic Hydrolysis of Human Milk Oligosaccharides. The Molecular Mechanism of Bifidobacterium Bifidum Lacto-N-biosidase
Cuxart, Irene; Coines, Joan; Esquivias, Oriol; Faijes, Magda; Planas, Antoni (Planas Sauter); Biarnés Fontal, Xevi; Rovira, Carme (ACS Catalysis 2022, 12(8), 4737–4743, 2022)Bifidobacterium bifidum lacto-N-biosidase (LnbB) is a critical enzyme for the degradation of human milk oligosaccharides in the gut microbiota of breast-fed infants. Guided by recent crystal structures, ... -
Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases
Planas, Antoni (Planas Sauter); Biarnés Fontal, Xevi; Hamer, Stefanie Nicole; Cord-Landwehr, Stefan; Waegeman, Hendrik; Moerschbacher, Bruno Maria; Kolkenbrock, Stephan (Scientific Reports. Vol.5, 8716 (2015), 2015-03)Chitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. These properties may depend not only on the ... -
Expanding the chitin oligosaccharide portfolio by engineering NodC chitin synthases in Escherichia coli
Guidi, Chiara; Biarnés Fontal, Xevi; Planas, Antoni (Planas Sauter); De Mey, Marjan (Current Research in Biotechnology. 2024;8:100255, 2024-09-17)Synthetic biology greatly accelerated the building process of potential microbial cell factories for the production of industrially relevant compounds, e.g., chitooligosaccharides (COS) which have an ... -
Formation of a Covalent Adduct in Retaining β-Kdo Glycosyl-Transferase WbbB via Substrate-Mediated Proton Relay
Sağıroğlugil, Mert; Liao, Qinghua; Planas, Antoni (Planas Sauter); Rovira, Carme (ChemCatChem. 2024;16:e202400769, 2024-07-22)The GT99 domain of the membrane-anchored WbbB glycosyltransferase (WbbBGT99) catalyzes the transfer of 3-deoxy-D-manno-oct-2-acid (β-Kdo) to an O-antigen saccharide acceptor with retention of stereochemistry. ... -
Glycosidases and glycosynthases in enzymatic synthesis of oligosaccharides: an overview
Planas, Antoni (Planas Sauter); Faijes, Magda (Afinidad. Vol.59, n.500 (2002), p.295-313, 2002)Retaining glycosidases have been extensively studied for the synthesis of oligosaccharides and glycoconjugates as a convenient enzymatic approach to achieve the required regio- and stereoselectivity in ... -
Indolyl Septanoside Synthesis for In Vivo Screening of Bacterial Septanoside Hydrolases
Pote, Aditya R.; Pascual, Sergi; Planas, Antoni (Planas Sauter); Peczuh, Mark (International Journal of Molecular Sciences 2021; 22(9):4497, 2021-04-26)Building-up and breaking-down of carbohydrates are processes common to all forms of life. Glycoside hydrolases are a broad class of enzymes that play a central role in the cleavage of glycosidic bonds, ... -
Integrative structure determination reveals functional global flexibility for an ultra-multimodular arabinanase
Lansky, Shifra; Salama, Rachel; Biarnés Fontal, Xevi; Shwartstein, Omer; Schneidman-Duhovny, Dina; Planas, Antoni (Planas Sauter); Shoham, Yuval; Shoham, Gil (Communications Biology 2022, 5, 465, 2022)AbnA is an extracellular GH43 α-L-arabinanase from Geobacillus stearothermophilus, a key bacterial enzyme in the degradation and utilization of arabinan. We present herein its full-length crystal ... -
MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering
Melero-Cobo, Xavier; Gallemí, Marçal; Carnicer, Marc; Monte, Elena; Planas, Antoni (Planas Sauter); Leivar, Pablo (Physiologia Plantarum 2025, 177 (1), 2025-02)Photosynthetic microalgae are promising green cell factories for the sustainable production of high-value chemicals and biopharmaceuticals. The chloroplast organelle is being developed as a chassis for ... -
Novel α-mannose-functionalized poly(β-amino ester) nanoparticles as mRNA vaccines with increased antigen presenting cell selectivity in the spleen
González-Ríos, Nil; Artigues Cladera, Margalida; Guerra Rebollo, Marta; Planas, Antoni (Planas Sauter); Borrós i Gómez, Salvador; Faijes, Magda; Fornaguera Puigvert, Cristina (Journal of Materials Chemistry B, 2023-06-14)mRNA vaccination has emerged as a prominent therapy for the future of medicine. Despite the colossal advance in this technology and worldwide efficacy proof (ca. COVID vaccines), mRNA carriers still ... -
Plasmodium falciparum apicomplexan-specific glucosamine-6-phosphate n-acetyltransferase is key for amino sugar metabolism and asexual blood stage development
Planas, Antoni (Planas Sauter); Leivar Rico, Pablo; Chi, Jordi; Cova, Marta; De las Rivas, Matilde; Medina, Ana; Junqueira Borges, Rafael; Usón, Isabel; Hurtado-Guerrero, Ramón; Izquierdo, Luis (Molecular Biology and Physiology. Vol.11, n.5 (2020), e02045-20, 2020-10)ABSTRACT UDP-N-acetylglucosamine (UDP-GlcNAc), the main product of the hexosamine biosynthetic pathway, is an important metabolite in protozoan parasites since its sugar moiety is incorporated into ... -
Preparative scale production of recombinant human transthyretin for biophysical studies of protein-ligand and protein-protein interactions
Planas, Antoni (Planas Sauter); Arsequell, Gemma; Cotrina, Ellen Y.; Vilà, Marta; Nieto, Joan (International Journal of Molecular Sciences. Vol.21, n.24 (2020), 9640, 2020-12)Human transthyretin (hTTR), a serum protein with a main role in transporting thyroid hormones and retinol through binding to the retinol-binding protein, is an amyloidogenic protein involved in familial ... -
Structural-functional analysis reveals a specific domain organization in family GH20 hexosaminidases
Faijes, Magda; Val Cid, Cristina; Biarnés Fontal, Xevi; Planas, Antoni (Planas Sauter) (PLoS ONE. Vol.10, n.5 (2015), e0128075, 2015-05)Hexosaminidases are involved in important biological processes catalyzing the hydrolysis of N-acetyl-hexosaminyl residues in glycosaminoglycans and glycoconjugates. The GH20 enzymes present diverse ... -
Structure-function features of a mycoplasma glycolipid synthase derived from structural data integration, molecular simulations, and mutational analysis
Planas, Antoni (Planas Sauter); Biarnés Fontal, Xevi; Romero, Javier (Romero García); Francisco, Carles (PLoS One. Vol.8, n.12 (2013), e81990, 2013-12)Glycoglycerolipids are structural components of mycoplasma membranes with a fundamental role in membrane properties and stability. Their biosynthesis is mediated by glycosyltransferases (GT) that catalyze ... -
Substrate distortion in the Michaelis complex of Bacillus 1,3–1,4-β-glucanase: insight from first principles molecular dynamics simulations
Planas, Antoni (Planas Sauter); Biarnés Fontal, Xevi; Nieto, Joan; Rovira i Virgili, Carme (Journal of Biological Chemistry. Vol.281, n.3 (2006), p.1432–1441, 2006-01)The structure and dynamics of the enzyme-substrate complex of Bacillus 1,3–1,4-β-glucanase, one of the most active glycoside hydrolases, is investigated by means of Car-Parrinello molecular dynamics ... -
Targeting transthyretin in Alzheimer's disease: Drug discovery of small-molecule chaperones as disease-modifying drug candidates for Alzheimer's disease
Planas, Antoni (Planas Sauter); Cotrina, Ellen Y.; Santos, Luis Miguel; Rivas, Josep; Blasi, Daniel; Leite, José Pedro; Liz, Márcia A.; Busquets, Maria Antònia; Prohens, Rafel; Gimeno, Ana; Jiménez-Barbero, Jesús; Gales, Luis; Llop, Jordi; Quintana, Jordi; Cardoso, Isabel; Arsequell, Gemma (European Journal of Medicinal Chemistry, 2021-12-15)Transthyretin (TTR) has a well-established role in neuroprotection in Alzheimer's Disease (AD). We have setup a drug discovery program of small-molecule compounds that act as chaperones enhancing ...